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<tr 134>
<ps "heading 1" 1><pn "<:t>1" 1><:crmk 1>
<:hr>Installing Breeze Software
<ps "Normal" 2>The Waters<:fc 1>‚<:/fc> <:imk 1>Breeze™ system software provides a single user interface for acquiring, processing, and reporting chromatographic data.
<imk 1>
<ie>Breeze system
<il>overview
</imk>
<ps "Normal" 2>For best results, your Breeze system should include, at a minimum, the following hardware:
<ps "bullet list multi" 3><pn "•<:t>" 2><:fc 2>Solvent delivery<:/fc> – Waters 1500 Series HPLC Pump (1525 or 1515)
<ps "bullet list multi" 3><pn "•<:t>" 2><:fc 2>Injector<:/fc> – Waters 717plus Autosampler with optional heater/chiller and/or a manual injector
<ps "bullet list multi" 3><pn "•<:t>" 2><:fc 2>Detector<:/fc> – Waters 2487 UV Detector and/or 2410 RI Detector; Waters SAT/IN™ Module (optional)
<ps "bullet list multi" 3><pn "•<:t>" 2><:fc 2>Column<:/fc> – HPLC or GPC column
<ps "bullet list multi" 3><pn "•<:t>" 2><:fc 2>Column heater<:/fc> – one zone (optional)
<ps "bullet list multi" 3><pn "•<:t>" 2><:fc 2>Computer<:/fc> (see <:xr "Table<:hs>1-1" 1>)
<ps "note" 4><:cs "Note" 1>Note:<:/cs> Use the <:fc 3>Breeze System Quick Install Guide<:/fc> to connect, install, and power on the instruments in the Breeze system, including the computer that runs the Breeze software.
<ps "heading 2" 5><pn "1.1  " 3>Requirements
<ps "Normal" 2>To install the <:imk 2>Breeze system software, you need the minimum configuration of computer software and hardware listed in <:xr "Table<:hs>1-1" 1>.
<imk 2>
<ie>Breeze system
<il>hardware requirements
<ie>Breeze system
<il>software requirements
<ie>Hardware requirements
<ie>Software requirements
<ie>Requirements
<il>hardware
<ie>Requirements
<il>software
</imk>
<tb 62>
<tbt>
<ps "tbl caption wide" 6><pn "Table<:hs>1-1  " 4><:fc 4><:crmk 2>Breeze System Software and Hardware Requirements<:v "<:cs "Emphasis"> (Continued)<:/cs>" 1><:/fc>
</tbt>
<tbh>
<row 1>
<ct 1>
<ps "tbl head" 7>Component
</ct>
<ct 1>
<ps "tbl head" 7>Minimum Configuration
</ct>
</row>
</tbh>
<tbb>
<row 1>
<ct 1>
<ps "tbl text" 8>Computer (with keyboard and mouse)
</ct>
<ct 1>
<ps "tbl text" 8>Pentium<:fc 1>‚<:/fc> II 233 MHz computer (or equivalent)
</ct>
</row>
<row 2>
<ct 1>
<ps "tbl text" 8>Operating system and graphical user interface
</ct>
<ct 1>
<ps "tbl text" 8>Windows NT<:fc 1>‚<:/fc> version 4.0, Service Pack 6a
</ct>
</row>
<row 1>
<ct 1>
<ps "tbl text" 8>Hard disk drive
</ct>
<ct 1>
<ps "tbl text" 8>4.0 GB
</ct>
</row>
<row 1>
<ct 1>
<ps "tbl text" 8>Diskette drive
</ct>
<ct 1>
<ps "tbl text" 8>3.5-inch, 1.44<:hs>MB
</ct>
</row>
<row 1>
<ct 1>
<ps "tbl text" 8>Random access memory (RAM)
</ct>
<ct 1>
<ps "tbl text" 8>64 MB
</ct>
</row>
<row 1>
<ct 1>
<ps "tbl text" 8>Monitor<:fn 63>
<fn 63>
<ps "table footnote" 9>For optimal display of the Breeze software, set the monitor resolution to 1024 x 768 pixels, small fonts, and at least High Color (64-bit). Lower resolutions may affect the appearance of the Breeze software.
</fn>
</ct>
<ct 1>
<ps "tbl text" 8>SVGA (1024 <:fc 5>¥<:/fc> 768 pixels)
</ct>
</row>
<row 1>
<ct 1>
<ps "tbl text" 8>Graphics capability
</ct>
<ct 1>
<ps "tbl text" 8>1024 <:fc 5>x<:/fc> 768, 64K colors
</ct>
</row>
<row 1>
<ct 1>
<ps "tbl text" 8>Printer
</ct>
<ct 1>
<ps "tbl text" 8>Any printer supported by Windows NT 4.0
</ct>
</row>
<row 1>
<ct 1>
<ps "tbl text" 8>CD-ROM drive
</ct>
<ct 1>
<ps "tbl text" 8>4x CD-ROM
</ct>
</row>
<row 2>
<ct 1>
<ps "tbl text" 8>Control card
</ct>
<ct 1>
<ps "tbl text" 8>Waters <:cs "Emphasis" 2>PCI <:/cs>Bus Laboratory Acquisition and Control/Environment (busLAC/E™) card<:fn 64>
<fn 64>
<ps "table footnote" 9>Install the PCI busLAC/E card according to the instructions in the <:fc 6>Breeze System Quick Install Guide<:/fc>.
</fn>
</ct>
</row>
<row 1>
<ct 1>
<ps "tbl text" 8>Serial SAT/IN module
</ct>
<ct 1>
<ps "tbl text" 8>Optional
</ct>
</row>
</tbb>
</tb>
</tr>
</page>
<page "2">
</page>
<page "3">
<tr 136>
<ps "Normal" 10><:xr "Figure<:hs>1-1" 2> shows the steps for installation and initial startup of the Breeze system.
<ps "figure" 11>
<af 36>
<tr 38>
<ps "Body" 12>Install the Breeze software
<ps "Body" 12>(see <:xr "Section<:hs>1.2, Setting IEEE-488 Addresses" 3>, <:xr "Section<:hs>1.3, Starting the Breeze System" 4>, 
<ps "Body" 12><:xr "Section<:hs>1.4, Installing Breeze Software" 5>, and 
<ps "Body" 12><:xr "Section<:hs>1.5, Starting the Breeze Software" 6>)
</tr>
<tr 48>
<ps "Body" 12>Make a single test injection (see <:xr "Section<:hs>2.2, Making a Single Injection" 7>)
</tr>
<tr 40>
<ps "Body" 12>Create, update, and run a sample set (see <:xr "Section<:hs>2.3.3, Acquiring Data with a Sample Set Method" 8>)
</tr>
<tr 42>
<ps "Body" 12>Ensure that all instruments appear on the Breeze Diagnostic/Configuration wizard (see <:xr "Section<:hs>1.6, Scanning Connected Instruments" 9>)
</tr>
<tr 44>
<ps "Body" 12>Acquire and process sample data, review sample data, and preview and print a report (see <:xr "Chapter<:hs>3, Processing the Data" 10>)
</tr>
<tr 46>
<ps "Body" 12>Prepare solvents and samples, and prime and/or purge the pump (see <:xr "Section<:hs>2.1, Preparing for Operation" 11>)
</tr>
</af>
<ps "fig caption wide" 13><pn "Figure<:hs>1-1  " 5><:crmk 3>Breeze System Startup Flow Diagram
<ps "heading 2" 5><pn "1.2  " 6><:crmk 4>Setting IEEE-488 Addresses
<ps "Normal" 2>To set <:imk 3>IEEE-488 addresses of the instruments in the Breeze system, refer to <:xr "Table<:hs>1-2" 12>. 
<imk 3>
<ie>IEEE-488 addresses, setting
<ie>Setting
<il>IEEE-488 address
<ie>Instruments
<il>IEEE-488 addresses
</imk>
<tb 68>
<tbt>
<ps "tbl caption wide" 6><pn "Table<:hs>1-2  " 7><:crmk 5>Setting IEEE-488 Addresses<:v "<:cs "Emphasis"> (Continued)<:/cs>" 1>
</tbt>
<tbh>
<row 1>
<ct 1>
<ps "tbl head" 7>Instrument
</ct>
<ct 1>
<ps "tbl head" 7>Reference
</ct>
</row>
</tbh>
<tbb>
<row 2>
<ct 1>
<ps "tbl text" 8>Waters 1500 Series HPLC Pump
</ct>
<ct 1>
<ps "tbl text" 8>See Section 2.3, Making Electrical Connections, in the <:fc 6>Waters 1500 Series HPLC Pump Installation and Maintenance Guide<:/fc>.
</ct>
</row>
<row 3>
<ct 1>
<ps "tbl text" 8>Waters 2487 Dual <:cs "Symbol" 3>l <:/cs>Absorbance Detector
</ct>
<ct 1>
<ps "tbl text" 8>See Section 2.6, Making Signal Connections, Section 2.7, Connecting to Other Devices, and Section 3.2.6, Operating Other Detector Functions, in the <:cs "Emphasis" 4>Waters 2487 Dual<:/cs><:cs "Symbol" 3> l <:/cs><:cs "Emphasis" 5>Absorbance Detector Operator’s Guide<:/cs>.
</ct>
</row>
<row 2>
<ct 1>
<ps "tbl text" 8>Waters 2410 Refractometer
</ct>
<ct 1>
<ps "tbl text" 8>See Section 3.2, Making IEEE-488 Signal Connections, in the <:fc 6>Waters 2410 Refractometer Operator’s Guide<:/fc>.
</ct>
</row>
<row 4>
<ct 1>
<ps "tbl text" 8>Waters 717plus Autosampler
</ct>
<ct 1>
<ps "tbl text" 8>See Section 2.5, Connecting Communication Cables, and
<:hr>Section 3.6, Configuring the Autosampler, in the <:fc 6>Waters 717plus Autosampler Operator’s Guide<:/fc>.
</ct>
</row>
</tbb>
</tb>
</tr>
</page>
<page "4">
<tr 137>
<ps "heading 2" 5><pn "1.3  " 8>Starting <:crmk 6>the Breeze System
<ps "Normal" 10>To properly start and operate the <:imk 4>Breeze system, always power on the components in the following order:
<imk 4>
<ie>Breeze system
<il>components
<ie>Powering on Breeze system components
</imk>
<ps "numb list" 14><pn "<:t>1.<:t>" 9>Power on each chromatographic instrument controlled by the IEEE<:hh>488 bus. Wait approximately 5 minutes to allow the internal calibration and diagnostic tests to run. If necessary, calibrate the detector(s).
<ps "ind note" 15><:fc 7>Note:<:/fc> You cannot run Breeze methods or sample sets unless all devices on the IEEE-488 bus that are assigned to the Breeze system are powered on and have passed any tests.
<ps "numb list+" 16><pn "<:t>2.<:t>" 10>If applicable, power on the SAT/IN module, then power on any instruments connected to the SAT/IN module.
<ps "numb list+" 16><pn "<:t>3.<:t>" 11>Power on all equipment not controlled by the <:fc 8>Breeze<:/fc> system software, such as computer peripherals (for example, printers).
<ps "numb list+" 16><pn "<:t>4.<:t>" 12>Power on the Breeze system computer.
<ps "numb list+" 17><pn "<:t>5.<:t>" 13>Log on to Windows NT:
<ps "alpha list" 18><pn "<:t>a.<:t>" 14>Press <:cs "Command" 6>Ctl-Alt-Del<:/cs>. The Login information dialog box appears.
<ps "alpha list+" 19><pn "<:t>b.<:t>" 15>Enter a user name and password, then click <:cs "Command" 6>OK<:/cs>. If you do not know your user name, password, or domain, see your system administrator. The desktop appears.
<ps "heading 2" 5><pn "1.4  " 16><:crmk 7>Installing Breeze Software
<ps "Normal" 2>To <:imk 5>install Breeze software:
<imk 5>
<ie>Installing Breeze software
<ie>Software, installing
</imk>
<ps "numb list" 14><pn "<:t>1.<:t>" 9>Insert the Breeze CD into the CD-ROM drive on the computer.
<ps "numb list+" 16><pn "<:t>2.<:t>" 10>Read the Introductory pages with copyrights, the Software License Agreement, and the Read Me First information.
</tr>
</page>
<page "5">
<tr 138>
<ps "numb list+" 16><pn "<:t>3.<:t>" 11>Click <:cs "Command" 6>Yes<:/cs> to install the common DLLs, if appropriate.
<ps "numb list+" 16><pn "<:t>4.<:t>" 12>Enter your Name, Company (Name), Serial (Number) on the Product Support Registration page, then click <:cs "Command" 6>Next<:/cs>.
<ps "numb list+" 16><pn "<:t>5.<:t>" 13>Click <:cs "Command" 6>Yes<:/cs> to confirm the registration information.
<ps "numb list+" 16><pn "<:t>6.<:t>" 17>Select the drive on which to install Breeze software, then click <:cs "Command" 6>Next<:/cs>.
<ps "numb list+" 16><pn "<:t>7.<:t>" 18>Select <:cs "Command" 6>LC and GPC<:/cs> as the default project<:cs "Bold" 7>, <:/cs>then click <:cs "Command" 6>Next<:/cs>.
<ps "numb list+" 16><pn "<:t>8.<:t>" 19>Click <:cs "Command" 6>Yes<:/cs> to confirm installation.
<ps "numb list+" 16><pn "<:t>9.<:t>" 20>If a previous version of the busLAC/E drivers is detected, click <:cs "Command" 6>Yes<:/cs> if you want to restore the original busLAC/E.ini file.
<ps "numb list, no number" 20><pn "<:t><:t>" 21>The Breeze Setup window shows the progress of the installation. Allow about 10 minutes for installation.
<ps "numb list+" 16><pn "<:t>10.<:t>" 22>When setup is complete, reboot the system, then click <:cs "Command" 6>Finish<:/cs>.
<ps "numb list+" 16><pn "<:t>11.<:t>" 23>Install the Breeze Key diskette in the diskette drive to enable use of the Breeze system:
<ps "alpha list" 18><pn "<:t>a.<:t>" 14>Run <:cs "Command" 6>A:\Setup<:/cs>.
<ps "alpha list+" 19><pn "<:t>b.<:t>" 15>Follow the instructions.
<ps "heading 2" 5><pn "1.5  " 24><:crmk 8>Starting the Breeze Software
<ps "note" 21><:cs "Note" 1>Note:<:/cs> If the Breeze system includes a SAT/IN module, ensure that the SAT/IN module and all instruments connected to it are powered on and have passed any startup diagnostics.
<ps "Normal" 10>To start the Breeze application:
<ps "numb list" 22><pn "<:t>1.<:t>" 9><:fc 9>Click <:imk 6><:/fc><:cs "Command" 6>Start<:/cs><:fc 9> (at the bottom of the desktop), select <:/fc><:cs "Command" 6>Programs<:/cs><:fc 9>, select <:/fc><:cs "Command" 6>Breeze<:/cs><:fc 9>, then select <:/fc><:cs "Command" 6>Breeze<:/cs><:fc 10> <:/fc><:fc 9>from the cascade menu<:/fc>.
<imk 6>
<ie>Starting Breeze system
<ie>Breeze system
<il>starting
</imk>
<ps "ind note" 15><:fc 7>Note: <:/fc>When the Breeze system is powered on for the first time after software installation, the Breeze Diagnostic/Configuration wizard starts, leading you through the configuration of the attached instruments (see <:xr "Section<:hs>1.6, Scanning Connected Instruments" 13>).
<ps "numb list+" 16><pn "<:t>2.<:t>" 10>The Breeze system startup routine begins. The Breeze software automatically initializes (boots) the PCI busLAC/E card and scans all system hardware components connected to the busLAC/E. After the PCI busLAC/E boots successfully, the software scans the IEEE-488 bus, then displays the <:imk 7>Breeze Diagnostic/Configuration wizard (<:xr "Figure<:hs>1-2" 14>).
<imk 7>
<ie>Breeze Diagnostic/Configuration wizard
<ie>Wizards
<il>Breeze Diagnostic/Configuration
</imk>
</tr>
</page>
<page "6">
<tr 139>
<ps "figure" 11>
<af 34>
<tr 35>
<ps "Body" 12>Instrument List
</tr>
</af>
<ps "fig caption" 23><pn "Figure<:hs>1-2  " 25><:crmk 9>Breeze Diagnostic/Configuration Wizard
<ps "numb list+" 16><pn "<:t>3.<:t>" 11>Ensure that the instruments in the list are powered on and have passed their start-up diagnostics. If any instruments are connected to the Breeze system but do not appear in the instrument list, click <:cs "Command" 6>Re-Scan Instruments<:/cs>.
</tr>
</page>
<page "7">
<tr 140>
<ps "numb list+" 17><pn "<:t>4.<:t>" 12>Click <:cs "Command" 6>Finish<:/cs>. The <:imk 8>Breeze software appears (<:xr "Figure<:hs>1-3" 15>).
<imk 8>
<ie>Sample Queue workspace
<ie>Workspaces
<il>Sample Queue
</imk>
<ps "figure" 11>
<af 6>
<tr 7>
<ps "Body" 12>Waters Breeze Help
</tr>
</af>
<ps "figure" 11>
<ps "fig caption" 23><pn "Figure<:hs>1-3  " 26><:crmk 10>First Screen of Breeze Software 
</tr>
</page>
<page "8">
<tr 141>
<ps "numb list+" 17><pn "<:t>5.<:t>" 13>Click-drag or minimize the Waters Breeze Help window to show the <:imk 9>Sample Queue workspace (<:xr "Figure<:hs>1-4" 16>).
<imk 9>
<ie>Sample Queue workspace
<ie>Workspaces
<il>Sample Queue
</imk>
<ps "figure" 11>
<af 13>
<tr 14>
<ps "Body" 12>Command Bar
</tr>
<tr 16>
<ps "Body" 12>Workspace
</tr>
<tr 17>
<ps "Body" 12>Acquisition Bar
</tr>
</af>
<ps "figure" 11>
<ps "fig caption" 23><pn "Figure<:hs>1-4  " 27><:crmk 11>Sample Queue Workspace
<ps "numb list+" 16><pn "<:t>6.<:t>" 17>Click <:cs "Command" 6>Manage Breeze<:/cs> on the command bar, then click <:cs "Command" 6>Projects<:/cs>. The Default project appears (<:xr "Figure<:hs>1-5" 17>). 
</tr>
</page>
<page "9">
<tr 142>
<ps "figure" 11>
<af 27>
<tr 28>
<ps "Body" 24><:fc 11> Default Project<:/fc><:cs "Callout" 8><:/cs>
</tr>
</af>
<ps "fig caption" 23><pn "Figure<:hs>1-5  " 28><:crmk 12>Default Project in the Manage Breeze Projects View
<ps "note" 4><:fc 12>Note:<:/fc> You can click the <:cs "BoldEmphasis" 9>How To<:/cs> button in the command bar to access many Breeze functions directly (see <:xr "Section<:hs>1.7, Using the Waters Breeze Help" 18>).
<ps "Normal" 10>For more information on:
<ps "bullet list multi" 3><pn "•<:t>" 2>Starting the Breeze system, see the Starting the Breeze System topic in the <:fc 6>Waters Breeze Help<:/fc> Find tab.
<ps "bullet list multi" 3><pn "•<:t>" 2>Adding an instrument to the Breeze system, see the Adding Instruments to the Breeze System topic in the <:fc 6>Waters Breeze Help<:/fc> Find tab.
<ps "bullet list multi" 3><pn "•<:t>" 2>Configuring instruments in the Breeze system, see the Configuring Instruments in the Breeze System topic in the <:fc 6>Waters Breeze Help<:/fc> Find tab.
<ps "bullet list multi" 3><pn "•<:t>" 2>Scanning the PCI busLAC/E for Breeze system instruments, see the Scanning the PCI busLAC/E Card for Breeze Instruments topic in the <:fc 6>Waters Breeze Help <:/fc>Find tab.
</tr>
</page>
<page "10">
<tr 143>
<ps "heading 2" 5><pn "1.6  " 29><:crmk 13>Scanning Connected Instruments
<ps "Normal" 10>To verify that <:fc 9>the instruments in your chromatographic system and the Breeze software are <:imk 10>connected<:/fc>:
<imk 10>
<ie>Connections, verifying
<ie>Verifying connections
<ie>Instruments
<il>connected
</imk>
<ps "numb list" 22><pn "<:t>1.<:t>" 9>Click <:cs "Command" 6>Manage Breeze<:/cs> on the command bar, then click <:cs "Command" 6>System<:/cs>. The <:imk 11>Manage Breeze workspace appears (<:xr "Figure<:hs>1-6" 19>).
<imk 11>
<ie>Manage Breeze System workspace
<ie>Workspaces
<il>Manage Breeze System
</imk>
<ps "figure" 11><:iaf 32>
<ps "fig caption" 23><pn "Figure<:hs>1-6  " 30>Manage Breeze Workspace<:crmk 14>
<ps "numb list, no number" 20><pn "<:t><:t>" 21>The Breeze instruments and their firmware revisions (PROM Version) are displayed. <:fc 9>A red circle with a diagonal line indicates the absence of the instrument or of the connection. The instruments are <:/fc>as follows:
<ps "ind list multi" 25><pn "•<:t>" 2><:fc 2>Solvent Delivery<:/fc> – Waters 1500 Series HPLC Pump
<ps "ind list multi" 25><pn "•<:t>" 2><:fc 2>Injector<:/fc> – Waters 717plus Autosampler or Manual Injector
<ps "ind list multi" 25><pn "•<:t>" 2><:fc 2>Column Heater<:/fc> – An attachment to the 1500 Series HPLC Pump (optional)
<ps "ind list multi" 25><pn "•<:t>" 2><:fc 2>UV Det<:/fc> – Waters 2487 Dual <:cs "Symbol" 3>l<:/cs> Absorbance Detector
<ps "ind list multi" 25><pn "•<:t>" 2><:fc 2>RI Det <:/fc>– Waters 2410 Refractive Index Detector
<ps "ind list multi" 25><pn "•<:t>" 2><:fc 2>External<:/fc> – Waters SAT/IN Module (optional) connecting non-IEEE-488 instruments to the Breeze system
<ps "numb list+" 26><pn "<:t>2.<:t>" 10>To add or remove instruments from the system, click <:cs "Command" 6>Diagnostics/Re-Configure<:/cs>.
<ps "numb list, no number" 20><pn "<:t><:t>" 21>For more information, see the Adding Instruments to the Breeze System topic in the <:fc 6>Waters Breeze Help<:/fc> Find tab.
</tr>
</page>
<page "11">
<tr 144>
<ps "numb list+" 16><pn "<:t>3.<:t>" 11>If the No BusLAC/E Detected dialog box appears and the system has a busLAC/E, click <:cs "Command" 6>Diagnose BusLAC/E installation problems<:/cs>, then click <:cs "Command" 6>OK<:/cs>. 
<ps "numb list+" 16><pn "<:t>4.<:t>" 12>Follow the steps in the Breeze Diagnostic/Configuration wizard.
<ps "numb list+" 16><pn "<:t>5.<:t>" 13>Click <:cs "Command" 6>Finish<:/cs> to reboot the system.
<ps "heading 2" 5><pn "1.7  " 31><:crmk 15>Using the Waters Breeze Help
<ps "Sidehead1" 27>Accessing Help Topics
<ps "Normal" 2>The <:fc 6>Waters <:imk 12>Breeze Help<:/fc> is installed with the Breeze software and provides instant access to information about the Breeze system as you use the software. You can access this Help from the Breeze Main window Help menu. This menu has the following commands:
<imk 12>
<ie>Breeze Help
<il>accessing
<ie>Help
<il>accessing
<ie>Breeze Help
<il> <:bb "nopage"><:cs "Italic">see also<:/cs> Help
</imk>
<ps "bullet list multi" 3><pn "•<:t>" 2><:fc 2>Breeze Help Topics<:/fc> – Activates the Breeze Help Topics browser, displaying the Contents, Index, and Find tabs. The Breeze Help Topics browser allows you to display and navigate all Breeze Help topics, and provides search and print capabilities.
<ps "bullet list multi" 3><pn "•<:t>" 2><:fc 2>Help On Breeze<:/fc> – Activates Help for the current Breeze window (same as pressing F1).
<ps "bullet list multi" 3><pn "•<:t>" 2><:fc 2>About Breeze<:/fc> – Identifies the Breeze software application with pertinent information about the Breeze software, system resource information, and altering user information.
<ps "note" 4><:fc 12>Note: <:/fc>For optimal display of the <:fc 3>Waters Breeze Help<:/fc>, set your monitor resolution to 1024 x 768 pixels, small fonts, and at least High Color (64-bit). This is also the recommended resolution for using the Breeze software. Lower resolutions may affect the appearance of the <:fc 3>Waters Breeze Help<:/fc>.
<ps "Normal" 10>In the Breeze Help Topics browser, click:
<ps "bullet list multi" 3><pn "•<:t>" 2><:fc 2>Contents<:/fc> – To display a table of contents of <:cs "Italic" 10>Waters <:/cs><:fc 6>Breeze Help<:/fc> topics, similar to the table of contents in any document. If you click the book icon, a series of books or procedures appears in the Contents window. Double-click the appropriate topic to display Help.
<ps "bullet list multi" 3><pn "•<:t>" 2><:fc 2>Index<:/fc> – To display an index of all topics in the <:fc 6>Waters Breeze Help<:/fc>. Type the first few letters of the index entry in the Index text box. The index displays the desired topic or subtopics. Double-click the appropriate topic to display Help.
<ps "bullet list multi" 3><pn "•<:t>" 2><:fc 2>Find<:/fc> – To search for a unique text string in the <:cs "Italic" 10>Waters<:/cs> <:fc 6>Breeze Help<:/fc> topics list. Type the letters, word, or phrase that you want to search for. A list of topics containing the letters, word, or phrase appears in the Find window. Double-click the appropriate topic to display Help.
<ps "Sidehead1" 27>Using Context-Sensitive Help
<ps "Normal" 2>You can access <:imk 13>context-sensitive Help from every window, workspace, dialog box, and tab in the Breeze software.
<imk 13>
<ie>Context-sensitive Help
<ie>Help
<il>context-sensitive
</imk>
</tr>
</page>
<page "12">
<tr 145>
<ps "Normal" 2><:fc 2>In the Breeze workspace <:/fc>– Click <:iaf 1> (Help) at the top of the Breeze workspace to activate context-sensitive Help. Then click the parameter, field, button, drop-down list, or other item for which you want information. A context-sensitive pop-up window explains the purpose of the item.
<ps "Normal" 2><:fc 2>In dialog boxes and tabs<:/fc> – Use one of these methods:
<ps "bullet list multi" 3><pn "•<:t>" 2>Click <:iaf 2> (Help) in the title bar of the dialog box or tab, then click the parameter, field, button, drop-down list, or other item for which you want information.
<ps "bullet list multi" 3><pn "•<:t>" 2>Right-click the item for which you want information, then click <:cs "Command" 6>What’s This?<:/cs>.
<ps "Normal" 2>If context-sensitive Help exists for the selected item, a <:imk 14>pop-up window appears, explaining the purpose of the item.
<imk 14>
<ie>Pop-up windows
<ie>Windows
<il>pop-up
</imk>
<ps "note" 4><:fc 12>Note:<:/fc> Context-sensitive Help is activated for only one item at a time. You must activate context-sensitive Help separately for each item or area in the window, workspace, dialog box, or tab for which you want information. To reactivate the context-sensitive Help tool and close the pop-up window, click the tool again. To close the pop-up window only, click anywhere else.
<ps "Sidehead1" 27>Using How To Help
<ps "Normal" 10>The <:imk 15>How To window is displayed automatically at Breeze startup (<:xr "Figure<:hs>1-7" 20>). It offers assistance for major procedures.
<imk 15>
<ie>How To Help
<ie>Help
<il>How To topics
</imk>
<ps "figure" 11>
<af 4>
<tr 5>
<ps "Body" 12>Chiclet
</tr>
</af>
<ps "fig caption" 28><pn "Figure<:hs>1-7  " 32><:crmk 16>How To Window
<ps "numb list" 22><pn "<:t>1.<:t>" 9>If the How To window is not open, click <:iaf 3> in the command bar.
<ps "numb list+" 16><pn "<:t>2.<:t>" 10>Click a <:iaf 9> (chiclet) for the procedure for which you want Help. The How To window shows a series of related topics. 
</tr>
</page>
<page "13">
<tr 146>
<ps "numb list+" 16><pn "<:t>3.<:t>" 11>Click a <:iaf 31> (shortcut button) to display the topic and to open the appropriate Breeze window or dialog box.
<ps "numb list+" 16><pn "<:t>4.<:t>" 12>Some topics display a See Also button under the title. Click <:cs "Command" 6>See Also<:/cs> for a list of related procedures, select a procedure, then click <:cs "Command" 6>Display<:/cs>. 
<ps "heading 2" 5><pn "1.8  " 33>Using the Tri-Pane Window
<ps "Normal" 10>The Breeze software displays a <:imk 16>tri-pane window consisting of the command bar, the workspace, and the acquisition bar (<:xr "Figure<:hs>1-8" 21>).
<imk 16>
<ie>Tri-pane windows
<ie>Windows
<il>tri-pane
</imk>
<ps "figure" 11>
<af 23>
<tr 24>
<ps "Body" 12>Command 
<ps "Body" 12>Bar
</tr>
<tr 26>
<ps "Body" 12>Workspace
</tr>
<tr 25>
<ps "Body" 12>Acquisition 
<ps "Body" 12>Bar
</tr>
</af>
<ps "fig caption" 28><pn "Figure<:hs>1-8  " 34><:crmk 17>Components of the Breeze Main Window
</tr>
</page>
<page "14">
<tr 147>
<ps "heading 3 (reset to 1)" 29><pn "1.8.1  " 35>Command Bar
<ps "Normal" 2>The <:imk 17>command bar displays the View Method, Find Data, View Data, Sample Queue, and Manage Breeze buttons (<:xr "Table<:hs>1-3" 22>). The command bar also contains the How To button and the Show Acquisition check box
<imk 17>
<ie>Command bar
<ie>Bars
<il>command
</imk>
<tb 65>
<tbt>
<ps "tbl caption" 30><pn "Table<:hs>1-3  " 36><:crmk 18>Command Button Functions<:v "<:cs "Emphasis"> (Continued)<:/cs>" 1>
</tbt>
<tbh>
<row 5>
<ct 2>
<ps "tbl head" 7>Button
</ct>
<ct 1>
<ps "tbl head" 7>
</ct>
<ct 1>
<ps "tbl head" 7>Description
</ct>
</row>
</tbh>
<tbb>
<row 6>
<ct 2>
<ps "figure" 11><:iaf 8>
</ct>
<ct 1>
<ps "tbl text" 8>
</ct>
<ct 1>
<ps "tbl text" 8>Displays the instrument, processing, and report/export parameters for the current method and allows you to create new methods.
</ct>
</row>
<row 7>
<ct 2>
<ps "figure" 11><:iaf 10>
</ct>
<ct 1>
<ps "tbl text" 8>
</ct>
<ct 1>
<ps "tbl text" 8>Displays data for the current project (only one project opens at a time). Use the buttons to select the desired data (Sample Set, Injections, Channels, Methods, Result Sets, Results, or Curves).
</ct>
</row>
<row 8>
<ct 2>
<ps "figure" 11><:iaf 11>
</ct>
<ct 1>
<ps "tbl text" 8>
</ct>
<ct 1>
<ps "tbl text" 8>Active only when data has been selected in the Find Data workspace or when data has been sent from the Real-Time plot.
</ct>
</row>
<row 9>
<ct 2>
<ps "figure" 11>
<ps "figure" 11><:iaf 18>
</ct>
<ct 1>
<ps "tbl text" 8>
</ct>
<ct 1>
<ps "tbl text" 8>Displays the Samples table or the Running table. Use the Samples table to create, modify, and load sample set methods. You can display and change data in a sample set method while it is running. The <:cs "Command" 6>Running<:/cs> table displays the sample set method as it runs.
</ct>
</row>
<row 9>
<ct 2>
<ps "figure" 11>
<ps "figure" 11><:iaf 20>
</ct>
<ct 1>
<ps "tbl text" 8>
</ct>
<ct 1>
<ps "tbl text" 8>Displays the Project button, where you create a new project, change a user or project, back up a project, or restore a project. The System button displays the system instrumentation (and version), accesses diagnostics, and provides the ability to update the system configuration.
</ct>
</row>
</tbb>
</tb>. 
</tr>
</page>
<page "15">
<tr 148>
<ps "heading 3" 31><pn "1.8.2  " 37>Workspace
<ps "Normal" 32>Pressing a command button changes the <:imk 18>workspace (<:xr "Table<:hs>1-4" 23>). When you start Breeze, the default workspace is the Samples table in Sample Queue. 
<imk 18>
<ie>Workspaces
<il>functions of
</imk>
<tb 67>
<tbt>
<ps "tbl caption" 33><pn "Table<:hs>1-4  " 38><:crmk 19>Workspace Functions<:v "<:cs "Emphasis"> (Continued)<:/cs>" 1>
</tbt>
<tbh>
<row 5>
<ct 1>
<ps "tbl head" 7>Workspace
</ct>
<ct 1>
<ps "tbl head" 7>Function
</ct>
<ct 1>
<ps "tbl head" 7>Description
</ct>
</row>
</tbh>
<tbb>
<row 10>
<ct 1>
<ps "tbl text" 8>View Method
</ct>
<ct 1>
<ps "tbl text" 8>Allows you to edit Breeze methods
</ct>
<ct 1>
<ps "tbl text" 8>Click one of the following three buttons:
<ps "tbl text" 8><:fc 2>Instrument<:/fc> <:fc 2>Parameters<:/fc> – Displays buttons for the selected instrument in the Breeze system. Click the appropriate button to display or change the properties.
<ps "tbl text" 8><:fc 2>Processing<:/fc> <:fc 2>Parameters <:/fc>– Displays buttons for the processing channels enabled in Instrument Parameters. Click the appropriate button to display or change the properties for each channel.
<ps "tbl text" 8><:fc 2>Report/Export Parameters <:/fc>– Displays report and export parameters for all channels. Click the appropriate button to display the report and/or export parameters for individual channels or click <:cs "Command" 6>All<:/cs> to select report/export parameters for all channels.
</ct>
</row>
<row 11>
<ct 1>
<ps "tbl text" 8>Find Data
</ct>
<ct 1>
<ps "tbl text" 8>Displays the Project workspace
</ct>
<ct 1>
<ps "tbl text" 8>A <:cs "Italic" 10>project<:/cs> is a collection of related methods, raw data channels, and results. Each project resides in the Breeze database under a single project name. The Find Data command displays data in a project by Sample Sets, Injections, Channels, Methods, Results Sets, Results, or Curves. You can process, review, preview, report, and/or export data. You can specify a filter for the data using the Filter By drop-down list. You can update (refresh) any data set using the Update button. (At least one injection or sample set must be acquired before you can use the Find Data command.) 
</ct>
</row>
<row 12>
<ct 1>
<ps "tbl text" 8>View Data
</ct>
<ct 1>
<ps "tbl text" 8>Displays the Review workspace
</ct>
<ct 1>
<ps "tbl text" 8>The View Data command displays data in the Review workspace after you select data in the Find Data workspace. You can preview the reports. The View Data command is disabled by default. 
</ct>
</row>
<row 12>
<ct 1>
<ps "tbl text" 8>Sample Queue
</ct>
<ct 1>
<ps "tbl text" 8>Displays the Samples and Running tables
</ct>
<ct 1>
<ps "tbl text" 8>The Sample Queue workspace is displayed by default at startup. You can set up a single sample or a group of samples for analysis in the Samples table of the Sample Queue workspace. You can also view the samples currently running in the Running table.
</ct>
</row>
<row 13>
<ct 1>
<ps "tbl text" 8>Manage Breeze
</ct>
<ct 1>
<ps "tbl text" 8>Allows you to manage Breeze users, projects, and systems
</ct>
<ct 1>
<ps "tbl text" 8>In the Manage Breeze workspace, you can:
<ps "ind list multi" 34><pn "•<:t>" 2>Create, delete, back up, or restore a project, and change a user or project.
<ps "ind list multi" 34><pn "•<:t>" 2>View the system hardware configurations, including PROM versions except for the 1500 Series Column Heater.
<ps "ind list multi" 34><pn "•<:t>" 2>Update the system configuration using diagnostics to rescan the PCI busLAC/E card.
</ct>
</row>
</tbb>
</tb>
</tr>
</page>
<page "16">
<tr 149>
<ps "heading 3" 31><pn "1.8.3  " 39>Acquisition Bar
<ps "Normal" 2>The <:imk 19>acquisition bar (<:xr "Figure<:hs>1-9" 24>) enables you to access acquisition functions (<:xr "Table<:hs>1-5" 25>), control solvent delivery (<:xr "Table<:hs>1-6" 26>), and see a real-time plot (<:xr "Table<:hs>1-7" 27>). After the Breeze system instruments are powered on and the software is started, the acquisition bar is active. 
<imk 19>
<ie>Acquisition bar
<ie>Bars
<il>Acquisition
</imk>
<ps "note" 4><:cs "Note" 1>Note:<:/cs> If the acquisition bar is not visible, select the <:fc 12>Show Acquisition<:/fc> check box in the command bar. To provide more workspace when needed, deselect the <:fc 12>Show Acquisition<:/fc> check box.
<ps "figure" 11>
<af 50>
<tr 54>
<ps "Body" 12>Solvent Delivery Controls
</tr>
<tr 53>
<ps "Body" 12>Acquisition Functions
</tr>
<tr 52>
<ps "Body" 12>Real-Time Plot
</tr>
</af>
<ps "fig caption" 28><pn "Figure<:hs>1-9  " 40><:crmk 20>Acquisition Bar (in an Idle System)
</tr>
</page>
<page "17">
<tr 150>
<ps "Normal" 2>
<tb 66>
<tbt>
<ps "tbl caption" 33><pn "Table<:hs>1-5  " 41><:crmk 21>Acquisition Bar - Acquisition Functions<:v "<:cs "Emphasis"> (Continued)<:/cs>" 1>
</tbt>
<tbh>
<row 5>
<ct 1>
<ps "tbl head" 7>Button
</ct>
<ct 1>
<ps "tbl head" 7>Function
</ct>
<ct 1>
<ps "tbl head" 7>Description
</ct>
</row>
</tbh>
<tbb>
<row 6>
<ct 1>
<ps "figure" 11><:iaf 12>
</ct>
<ct 1>
<ps "tbl text" 8>Make Single Injection
</ct>
<ct 1>
<ps "tbl text" 8>When you click this button, the Specify Single Inject Parameters dialog box appears. Enter the parameters for the single injection, then click <:cs "Command" 6>Inject<:/cs>.
</ct>
</row>
<row 9>
<ct 1>
<ps "figure" 11><:iaf 19>
<ps "figure" 11>
</ct>
<ct 1>
<ps "tbl text" 8>Run Sample Set
</ct>
<ct 1>
<ps "tbl text" 8>This button is enabled when the Breeze system is Idle and the Sample Queue workspace displays at least one row. When you click this button, the Run Sample Set dialog box appears. Enter the parameters for the sample set, then click <:cs "Command" 6>Run<:/cs>.
</ct>
</row>
<row 12>
<ct 1>
<ps "figure" 11><:iaf 21>
<ps "figure" 11>
</ct>
<ct 1>
<ps "tbl text" 8>Abort Run
</ct>
<ct 1>
<ps "tbl text" 8>When a single injection is running, this button stops the acquisition immediately. When a sample set is running, you can stop the set after the current injection is complete or stop immediately. 
</ct>
</row>
<row 14>
<ct 1>
<ps "figure" 11><:iaf 22>
</ct>
<ct 1>
<ps "tbl text" 8>Do Last
</ct>
<ct 1>
<ps "tbl text" 8>Click this button to repeat the most recent run (single injection or sample set method). 
</ct>
</row>
<row 9>
<ct 1>
<ps "figure" 11><:iaf 29>
</ct>
<ct 1>
<ps "tbl text" 8>Equilibrate System
</ct>
<ct 1>
<ps "tbl text" 8>Click this button to open the Equilibrate System dialog box. Select a method to use for equilibration, then click <:cs "Command" 6>Equilibrate<:/cs>. It initiates pump flow and displays a plot of the baseline in the real-time plot so you can monitor the baseline. Click <:cs "Command" 6>Abort Run <:/cs>to exit the baseline monitor.
</ct>
</row>
<row 15>
<ct 1>
<ps "figure" 11><:iaf 30>
<ps "figure" 11>
</ct>
<ct 1>
<ps "figure" 11>
<ps "figure" 11>
<ps "figure" 11>
<ps "figure" 11>
</ct>
<ct 1>
<ps "tbl text" 8>This part of the acquisition bar displays information for the samples currently being acquired: name of the sample set, name of the sample being injected, vial number, injection number, elapsed run time, and system status. 
<ps "tbl text" 8>The status bar provides the status of the Breeze system. When you first start Breeze software and during hardware initialization, a progress bar and a Cancel boot button also appear. If you select <:cs "Command" 6>Cancel Boot<:/cs>, the button changes to Initialize HW. Select <:cs "Command" 6>Initialize HW<:/cs> to boot your system. 
</ct>
</row>
</tbb>
</tb>
</tr>
<tr 152>
<ps "Body" 35><:fc 13>Sample Set Name
<:hr>Method Name
<:hr>Sample Name
<:hr>Vial/Injection/RunTime
<:hr><:/fc>
</tr>
<tr 153>
<ps "Body" 36>Status
</tr>
</page>
<page "18">
<tr 151>
<ps "Normal" 2>
<tb 69>
<tbt>
<ps "tbl caption" 33><pn "Table<:hs>1-6  " 42><:crmk 22>Acquisition Bar - Solvent Delivery Control<:v "<:cs "Emphasis"> (Continued)<:/cs>" 1>
</tbt>
<tbh>
<row 5>
<ct 1>
<ps "tbl head" 7>Button
</ct>
<ct 1>
<ps "tbl head" 7>Function
</ct>
<ct 1>
<ps "tbl head" 7>Description
</ct>
</row>
</tbh>
<tbb>
<row 16>
<ct 1>
<ps "figure" 11><:iaf 33>
</ct>
<ct 1>
<ps "tbl text" 8>Pump Ramp Time<:fn 70>
<fn 70>
<ps "TableFootnote" 37>The Pump Ramp Time button does not affect the Purge Wizard. During a purge, the flow always ramps to the target flow over one minute. When purging the Waters autosampler with the Purge wizard, the flow rate ramps to the combined flow rate set for each pump before the autosampler’s waste valve opens. Make sure that the purge flow rates will not damage the column. 
</fn>
</ct>
<ct 1>
<ps "tbl text" 8>Click this button to apply a pump flow rate ramp time of 1.00 minute. Right-click this button to select a ramp time other than 1.00 minute. 
<ps "tbl text" 8>When this button is enabled (has a lighter background in the down position), the flow rate ramps over the selected time, protecting your columns from rapid changes. 
<ps "tbl note" 38><:cs "Note" 1>Caution:<:/cs> When the button is not enabled (has a darker background in the up position), the pump immediately goes to the new setting. Ensure that your columns can tolerate rapid changes, or that they are temporarily removed from the flow. 
</ct>
</row>
<row 14>
<ct 1>
<ps "figure" 11><:iaf 49>
</ct>
<ct 1>
<ps "tbl text" 8>Purge Wizard
</ct>
<ct 1>
<ps "tbl text" 8>Click this button to prime and/or purge the pump, injector, and/or detector.
</ct>
</row>
<row 14>
<ct 1>
<ps "figure" 11><:iaf 55>
</ct>
<ct 1>
<ps "tbl text" 8>Stop Pump Flow
</ct>
<ct 1>
<ps "tbl text" 8>Click this button to stop pump flow immediately.
</ct>
</row>
<row 17>
<ct 1>
<ps "figure" 11><:iaf 56>
</ct>
<ct 1>
<ps "tbl text" 8>Solvent Delivery Controls<:fn 71>
<fn 71>
<ps "TableFootnote" 39>The maximum combined flow rate for the 1525 Binary HPLC Pump should not exceed 10.00 mL/min. Although Breeze software allows a total flow rate of up to 20.00 mL/min, when either pump A or B exceeds 10.00 mL/min, the pump may stall. 
</fn>
</ct>
<ct 1>
<ps "tbl text" 8>To change the current values, move the arrows next to the flow rate or composition scale, or type a value in the box below the display, then select <:cs "Command" 6>Go<:/cs>. If you do not select Go, the original values reappear after a short delay.
</ct>
</row>
<row 18>
<ct 1>
<ps "figure" 11><:iaf 57>
</ct>
<ct 1>
<ps "tbl text" 8>Pressure Gauge
</ct>
<ct 1>
<ps "tbl text" 8>Displays the current backpressure of the system and defines the pressure zone within the minimum and maximum pressure limits. To change the current values, move the arrows gauge, or type a value in the box below the display, then select <:cs "Command" 6>Go<:/cs>. If you do not select Go, the original values reappear after a short delay.
</ct>
</row>
</tbb>
</tb>
</tr>
</page>
<page "19">
<tr 154>
<ps "Normal" 2>
<tb 72>
<tbt>
<ps "tbl caption" 33><pn "Table<:hs>1-7  " 43><:crmk 23>Acquisition Bar - Real-Time Plot<:v "<:cs "Emphasis"> (Continued)<:/cs>" 1>
</tbt>
<tbh>
<row 5>
<ct 1>
<ps "tbl head" 7>Button
</ct>
<ct 1>
<ps "tbl head" 7>Function
</ct>
<ct 1>
<ps "tbl head" 7>Description
</ct>
</row>
</tbh>
<tbb>
<row 14>
<ct 1>
<ps "figure" 11><:iaf 58>
</ct>
<ct 1>
<ps "tbl text" 8>Plot Display
</ct>
<ct 1>
<ps "tbl text" 8>Click this button to select either overlaid or stacked plots for the real-time plot.
</ct>
</row>
<row 6>
<ct 1>
<ps "figure" 11><:iaf 59>
</ct>
<ct 1>
<ps "tbl text" 8>Plot Scaling
</ct>
<ct 1>
<ps "tbl text" 8>Click this button to show or hide plot scaling. By default, plot scaling is selected. When you deselect this button, the plot scales disappear and the real-time plot enlarges.
</ct>
</row>
<row 19>
<ct 1>
<ps "figure" 11><:iaf 60>
</ct>
<ct 1>
<ps "tbl text" 8>Strip Chart Mode
</ct>
<ct 1>
<ps "tbl text" 8>Click this button to display the most recent minutes of the chromatogram. Right-click <:cs "Command" 6>Strip Chart Mode<:/cs> to select the plot window width in a scrolling 10-minute window. Right-click the button to change the number of minutes. Deselect the button to show the entire chromatographic run.
</ct>
</row>
<row 12>
<ct 1>
<ps "figure" 11><:iaf 61>
</ct>
<ct 1>
<ps "tbl text" 8>Review
</ct>
<ct 1>
<ps "tbl text" 8>When an injection is running, you can click this button to display the real-time plot in the Review workspace. It updates Review with the data acquired from the currently running sample set method.
</ct>
</row>
</tbb>
</tb>
<ps "Normal" 2>
</tr>
</page>
<page "20">
</page>
</stf>